A SIMPLE 2-STEP PROCEDURE FOR ISOLATION OF C-REACTIVE PROTEIN

被引:26
作者
GANROT, PO
KINDMARK, CO
机构
[1] Department of Clinical Chemistry, Malmö General Hospital, Malmö
关键词
D O I
10.1016/0005-2795(69)90104-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
C-reactive protein is strongly adsorbed to BaSO4 precipitate and can be eluted from the precipitate by a 1.6 M solution of (NH4)2SO4. These features can be utilised in the elaboration of a simple technique for large-scale partial purification of C-reactive protein from serum or serous fluids. In the presence of Ca2+, C-reactive protein is specifically bound to agar gel from which it can be eluted by addition of EDTA. This binding is possibly analogous to the binding of C-reactive protein to the pneumococcal C-polysaccaride. Electrophoretically pure C-reactive protein was prepared from human serum and ascitic or pleural fluid by a combination of BaSO4 adsorption and agar gel adsorption with a recovery of about 70%. © 1969.
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页码:443 / &
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