RAPID SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES ON A GRAFTED POLYMER SUPPORT

被引：57

作者：

POTAPOV, VK ^{[1
]}

VEIKO, VP ^{[1
]}

KOROLEVA, ON ^{[1
]}

SHABAROVA, ZA ^{[1
]}

机构：

[1] MV LOMONOSOV STATE UNIV,BELOZERSKII MOLEC BIOL & BIOORGAN CHEM LAB,MOSCOW 117234,USSR

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 6卷 / 06期

关键词：

D O I：

10.1093/nar/6.6.2041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rapid synthesis of oligodeoxyribonucleotides is described by a solid phase method. Polyaterene grafted on the surface of polytetrafluoroethylene has been found to be an adequate support. The properties of the support were studied in the synthesis of a number of oligonucleotides 6 t o 15 nucleotides long.A flow-type semi-automatic synthesizer has been used. Each nucleotide addition involves seven steps and takes 8 hours. Oligonucleotide isolated and purified by ion-exchange chromatography amounts to 1-3 μmol per gram of support. © 1979 Information Retrieval Limited.

引用

页码：2041 / 2056

页数：16

共 17 条

[1] G-VALUES OF GAMMA-RAY INITIATION OF VINYL POLYMERIZATION AND THEIR RELATION TO GRAFT COPOLYMER FORMATION [J].

BALLANTINE, DS ;

GLINES, A ;

METZ, DJ ;

BEHR, J ;

MESROBIAN, RB ;

RESTAINO, AJ .

JOURNAL OF POLYMER SCIENCE, 1956, 19 (91) :219-224

[2] CHROMATOGRAPHY OF P-32-LABELLED OLIGONUCLEOTIDES ON THIN LAYERS OF DEAE-CELLULOSE [J].

BROWNLEE, GG ;

SANGER, F .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1969, 11 (02) :395-&

[3] STUDIES ON POLYNUCLEOTIDES .105. TOTAL SYNTHESIS OF STRUCTURAL GENE FOR AN ALANINE TRANSFER RIBONUCLEIC-ACID FROM YEAST - CHEMICAL SYNTHESIS OF AN ICOSADEOXYRIBONUCLEOTIDE CORRESPONDING TO NUCLEOTIDE SEQUENCE 31 TO 50 [J].

BUCHI, H ;

KHORANA, HG .

JOURNAL OF MOLECULAR BIOLOGY, 1972, 72 (02) :251-288

[4] RAPID SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES - NEW SOLID-PHASE METHOD [J].

GAIT, MJ ;

SHEPPARD, RC .

NUCLEIC ACIDS RESEARCH, 1977, 4 (04) :1135-1158

[5] RAPID SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES .2. MACHINE-AIDED SOLID-PHASE SYNTHESES OF 2 NONANUCLEOTIDES AND AN OCTANUCLEOTIDE [J].

GAIT, MJ ;

SHEPPARD, RC .

NUCLEIC ACIDS RESEARCH, 1977, 4 (12) :4391-4410

[6] ROUS-SARCOMA VIRUS GENOME IS TERMINALLY REDUNDANT - 5' SEQUENCE [J].

HASELTINE, WA ;

MAXAM, AM ;

GILBERT, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (03) :989-993

[7]

HASHMANN J, 1968, J AM CHEM SOC, V91, P2749

[8] DEOXYRIBOOLIGONUCLEOTIDE SYNTHESIS ON A POLYMER SUPPORT [J].

HAYATSU, H ;

KHORANA, HG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1966, 88 (13) :3182-&

[9]

KOSTER H, 1974, LIEBIGS ANN CHEM, P959

[10]

MISHENINA GF, 1978, BIOORG KHIM+, V4, P735

← 1 2 →