CD8+ T-CELLS IN PSORIATIC LESIONS PREFERENTIALLY USE T-CELL RECEPTOR V(BETA)3 AND/OR V(BETA)13.1 GENES

被引：206

作者：

CHANG, JCC ^{[1
]}

SMITH, LR ^{[1
]}

FRONING, KJ ^{[1
]}

SCHWABE, BJ ^{[1
]}

LAXER, JA ^{[1
]}

CARALLI, LL ^{[1
]}

KURLAND, HH ^{[1
]}

KARASEK, MA ^{[1
]}

WILKINSON, DI ^{[1
]}

CARLO, DJ ^{[1
]}

BROSTOFF, SW ^{[1
]}

机构：

[1] PSORIASIS RES INST,PALO ALTO,CA 94301

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 20期

关键词：

PSORIASIS; INFLAMMATION;

D O I：

10.1073/pnas.91.20.9282

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Psoriasis is an inflammatory skin disorder characterized by epidermal keratinocyte hyperproliferation in association with a cellular infiltrate. There is evidence that activated T cells play a role in psoriatic plaque formation. We examined the T-cell receptor beta-chain variable gene segment (V-beta) use of epidermal T cells in shave biopsies of psoriatic lesions. Our results show increased expression of V (beta)3 and/or V (beta)13.1 messages in the CD8(+), but not CD4(+), T cells in the lesions of a majority of patients studied. Sequence analysis of complementarity-determining region 3 (CDR3) of these two V (beta) genes from the skin demonstrated monoclonality or marked oligoclonality. A second biopsy from the same or different lesions, performed 3.5-8 months later in four patients, again revealed increased V (beta)3 and/or V (beta)13.1 expression and clonality. Moreover, in three of the four patients, the same V (beta) CDR3 rearrangement was found in both biopsies, although there was no V (beta) CDR3 homology between patients. In two patients in which V (beta)3 and/or V (beta)13.1 was not increased, an increase in V (beta)17 gene use and clonality was found. The clonality of V (beta) sequence data indicates these cells are recruited and expanded in situ. The persistence of V (beta)3-and/or V (beta)13.1-bearing CD8(+) T cells in lesions that did not undergo resolution suggests their role as effector cells rather than as regulatory cells.

引用

页码：9282 / 9286

页数：5

共 19 条

[1] PREFERENTIAL V-BETA-GENE USAGE AND LACK OF JUNCTIONAL SEQUENCE CONSERVATION AMONG HUMAN T-CELL RECEPTORS SPECIFIC FOR A TETANUS TOXIN DERIVED PEPTIDE - EVIDENCE FOR A DOMINANT ROLE OF A GERMLINE-ENCODED V-REGION IN ANTIGEN MAJOR HISTOCOMPATIBILITY COMPLEX RECOGNITION [J].