NEW PROCEDURE FOR THE PRODUCTION OF INFLUENZA VIRUS-SPECIFIC DOUBLE-STRANDED DNAS

被引：15

作者：

CATON, AJ

ROBERTSON, JS

机构：

[1] Division of Virology, Department of Pathology, University of Cambridge, Laboratories Block, Addenbrooke's Hospital, Cambridge, Hills Road

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 7卷 / 06期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1093/nar/7.6.1445

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel technique is described for the production of pure, full-length influenza virus ds DNA's corresponding to each segment of the influenza virus genome, and suitable for molecular cloning and restriction endonuclease mapping. The method involves the synthesis of DNA complementary to both virion (negative strand) and messenger (positive strand) RNA, gel purification and annealing. By avoiding the use of SI nuclease, which often removes the terminal regions of DNA duplexes, the method allows transcription of the total sequence information of influenza virion and messenger RNA's into a ds DNA form. © 1979 Information Retrieval Limited.

引用

页码：1445 / 1456

页数：12

共 29 条

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