PREPARATION OF MONOCLONAL-ANTIBODIES AGAINST BRAIN NATRIURETIC PEPTIDE AND THEIR APPLICATION TO RADIOIMMUNOASSAY AND PASSIVE-IMMUNIZATION

被引：4

作者：

ITOH, H ^{[1
]}

NAKAO, K ^{[1
]}

MUKOYAMA, M ^{[1
]}

SAITO, Y ^{[1
]}

YAMADA, T ^{[1
]}

SHIRAKAMI, G ^{[1
]}

ARAI, H ^{[1
]}

HOSODA, K ^{[1
]}

SUGA, S ^{[1
]}

YOSHIDA, I ^{[1
]}

IMURA, H ^{[1
]}

机构：

[1] KYOTO UNIV,SCH MED,DEPT MED,DIV 2,54 SHOGOIN KAWAHARA CHO,SAKYO KU,KYOTO 606,JAPAN

来源：

ENDOCRINOLOGY | 1990年 / 127卷 / 03期

关键词：

D O I：

10.1210/endo-127-3-1292

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Two monoclonal antibodies (mAbs) directed toward brain natriuretic peptide (BNP), KY-BNP-I and KYBNP- II, have been produced. Both mAbs against BNP possessed high affinities for BNP, with association constants (Ka) of 4.0 x 109M-1(KY-BNP-I) and 2.0 x 1010M-1(KY-BNP-II). With these mAbs, specific RIAs for BNP have been established. The least detectable quantities of BNP were 5 pg/tube (KY-BNP-I) and 1 pg/tube (KY-BNP-II). Cross-reactivities of α-human and rat atrial natriuretic polypeptides were less than 0.001%. These RIAs detected BNP-like immunoreactivity (BNP-LI) not only in the porcine brain but also in the canine brain, with the highest concentration in the medulla oblongata. These RIAs also detected BNP-LI in both the porcine and canine hearts and in the porcine plasma. The iv pretreatment of purified mAb[KY-BNPII] almost completely blocked the hypotensive action of iv administered BNP in rats, with the concomitant suppression of BNP-induced increase of the plasma cyclic GMP level. These results indicate that our mAbs against BNP will serve as a useful tool for the elucidation of the physiological and pathophysiological significance of BNP as a neuropeptide and as a hormone. © 1990 by The Endocrine Society.

引用

页码：1292 / 1300

页数：9

共 39 条

[1]

HAMET P, 1986, J HYPERTENS, V4, pS49

[2] PORCINE BRAIN NATRIURETIC PEPTIDE, ANOTHER MODULATOR OF BOVINE ADRENOCORTICAL STEROIDOGENESIS [J].

HASHIGUCHI, T ;

HIGUCHI, K ;

OHASHI, M ;

MINAMINO, N ;

KANGAWA, K ;

MATSUO, H ;

NAWATA, H .

FEBS LETTERS, 1988, 236 (02) :455-461

[3] BRAIN NATRIURETIC PEPTIDE INTERACTS WITH ATRIAL NATRIURETIC PEPTIDE RECEPTOR IN CULTURED RAT VASCULAR SMOOTH-MUSCLE CELLS [J].

HIRATA, Y ;

SHICHIRI, M ;

EMORI, T ;

MARUMO, F ;

KANGAWA, K ;

MATSUO, H .

FEBS LETTERS, 1988, 238 (02) :415-418

[4] ATRIAL NATRIURETIC POLYPEPTIDES - STRUCTURE ACTIVITY RELATIONSHIP IN THE CENTRAL ACTION - A COMPARISON OF THEIR ANTIDIPSOGENIC ACTIONS [J].

ITOH, H ;

NAKAO, K ;

KATSUURA, G ;

MORII, N ;

SHIONO, S ;

YAMADA, T ;

SUGAWARA, A ;

SAITO, Y ;

WATANABE, K ;

IGANO, K ;

INOUYE, K ;

IMURA, H .

NEUROSCIENCE LETTERS, 1987, 74 (01) :102-106

[5] CHRONIC BLOCKADE OF ENDOGENOUS ATRIAL NATRIURETIC POLYPEPTIDE (ANP) BY MONOCLONAL-ANTIBODY AGAINST ANP ACCELERATES THE DEVELOPMENT OF HYPERTENSION IN SPONTANEOUSLY HYPERTENSIVE AND DEOXYCORTICOSTERONE ACETATE-SALT HYPERTENSIVE RATS [J].

ITOH, H ;

NAKAO, K ;

MUKOYAMA, M ;

YAMADA, T ;

HOSODA, K ;

SHIRAKAMI, G ;

MORII, N ;

SUGAWARA, A ;

SAITO, Y ;

SHIONO, S ;

ARAI, H ;

YOSHIDA, I ;

IMURA, H .

JOURNAL OF CLINICAL INVESTIGATION, 1989, 84 (01) :145-154

[6] CENTRAL ACTIONS OF ATRIAL NATRIURETIC POLYPEPTIDES IN SPONTANEOUSLY HYPERTENSIVE AND NORMOTENSIVE RATS [J].

ITOH, H ;

NAKAO, K ;

MORII, N ;

SUGAWARA, A ;

YAMADA, T ;

SHIONO, S ;

SAITO, Y ;

MUKOYAMA, M ;

ARAI, H ;

SAKAMOTO, M ;

IMURA, H .

JAPANESE CIRCULATION JOURNAL-ENGLISH EDITION, 1987, 51 (10) :1208-1215

[7] ANTIDIPSOGENIC ACTION OF A NOVEL PEPTIDE, BRAIN NATRIURETIC PEPTIDE, IN RATS [J].

ITOH, H ;

NAKAO, K ;

YAMADA, T ;

SHIRAKAMI, G ;

KANGAWA, K ;

MINAMINO, N ;

MATSUO, H ;

IMURA, H .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1988, 150 (1-2) :193-196

[8] CENTRAL INTERACTION OF THE BRAIN ATRIAL NATRIURETIC POLYPEPTIDE (ANP) SYSTEM AND THE BRAIN RENIN-ANGIOTENSIN SYSTEM IN ANP SECRETION FROM HEART - EVIDENCE FOR POSSIBLE BRAIN HEART AXIS [J].

ITOH, H ;

NAKAO, K ;

YAMADA, T ;

MORII, N ;

SHIONO, S ;

SUGAWARA, A ;

SAITO, Y ;

MUKOYAMA, M ;

ARAI, H ;

IMURA, H .

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 1988, 66 (03) :255-261

[9] GAMMA-ATRIAL NATRIURETIC POLYPEPTIDE (GAMMA-ANP)-DERIVED PEPTIDES IN HUMAN-PLASMA - COSECRETION OF N-TERMINAL GAMMA-ANP FRAGMENT AND ALPHA-ANP [J].

ITOH, H ;

NAKAO, K ;

SUGAWARA, A ;

SAITO, Y ;

MUKOYAMA, M ;

MORII, N ;

YAMADA, T ;

SHIONO, S ;

ARAI, H ;

HOSODA, K ;

IMURA, H .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1988, 67 (03) :429-437

[10] POSSIBLE INVOLVEMENT OF CENTRAL ATRIAL NATRIURETIC POLYPEPTIDE IN REGULATION OF HYPOTHALAMO-PITUITARY-ADRENAL AXIS IN CONSCIOUS RATS [J].

ITOH, H ;

NAKAO, K ;

KATSUURA, G ;

MORII, N ;

YAMADA, T ;

SHIONO, S ;

SAKAMOTO, M ;

SUGAWARA, A ;

SAITO, Y ;

EIGYO, M ;

MATSUSHITA, A ;

IMURA, H .

NEUROSCIENCE LETTERS, 1986, 69 (03) :254-258

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