REGIONAL DISTRIBUTION OF THE AMPA GLUTAMATE-RECEPTOR SUBUNITS GLUR2(4) IN HUMAN HIPPOCAMPUS

被引：14

作者：

BLUMCKE, I

WOLF, HK

HOF, PR

MORRISON, JH

WIESTLER, OD

机构：

[1] MT SINAI SCH MED,FISHBERG RES CTR NEUROBIOL,NEW YORK,NY 10029

[2] MT SINAI SCH MED,DEPT GERIATR & ADULT DEV,NEW YORK,NY 10029

来源：

BRAIN RESEARCH | 1995年 / 682卷 / 1-2期

关键词：

EXCITATORY AMINO ACID; GLUTAMATE RECEPTOR; IMMUNOHISTOCHEMISTRY; DENTATE GYRUS; AMMONS HORN;

D O I：

10.1016/0006-8993(95)00354-S

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In order to characterize the regional and cellular distribution patterns of individual ionotropic excitatory amino acid receptor subunits in the human hippocampus we performed an immunohistochemical analysis using the monoclonal antibody 3A11 to the AMPA GluR2(4) subunit. The study was based on paraffin embedded hippocampal specimens of five human brains obtained at autopsy. GluR2(4) immunoreactivity was consistently higher in the hippocampus as compared to the adjacent areas of the mesial temporal lobe. Virtually all neurons showed intracytoplasmic staining of the perikarya and dendritic profiles with well defined laminar patterns. The most intense GluR2(4) immunoreactivity was observed in the target structures of messy fibers, thus indicating that GluR2(4) AMPA subunits may be involved in NMDA-independent synaptic transmission pathways and long-term potentiation. Glial cells were not immunoreactive. These findings may provide basic information for studies of the GluR2(4) subunit in human hippocampus during various neuropathological conditions, such as temporal lobe epilepsy, ischemia and Alzheimer's disease.

引用

页码：239 / 244

页数：6

共 35 条

[1]

Armstrong, Ikonomovic, Sheffield, Wenthold, AMPA-selective glutamate receptor subtype immunoreactivity in the entorhinal cortex of non-demented elderly and patients with Alzheimer's disease, Brain Res., 639, pp. 207-216, (1994)

[2]

Bekkers, Stevens, NMDA and non-NMDA receptors are co-localized at individual excitatory synapses in cultured rat hippocampus, Nature, 341, pp. 230-233, (1989)

[3]

Blackstone, Levey, Martin, Immunological detection of glutamate receptor subtypes in human central nervous system, Ann. Neurol., 31, pp. 680-683, (1992)

[4]

Bockers, Zimmer, Muller, Bergmann, Brose, Kreutz, Expression of the NMDAR1 receptor in selected human brain regions, NeuroReport, 5, pp. 965-969, (1994)

[5]

Boulter, Hollmann, O'Shea-Greenfield, Hartley, Deneris, Maron, Heinemann, Molecular cloning and functional expression of glutamate receptor subunit genes, Science, 249, pp. 1033-1037, (1990)

[6]

Choi, Glutamate neurotoxicity and diseases of the nervous system, Neuron, 1, pp. 623-634, (1988)

[7]

DeFelipe, Huntley, del Rio, Sola, Morrison, Microzonal decrease in the immunostaining for non-NMDA ionotropic excitatory amino acid receptor subunits GluR2/3 and gluR5/6/7 in the human epileptogenic neocortex, Brain Res., 657, pp. 150-158, (1994)

[8]

Eastwood, Burnet, Beckwith, Kerwin, Harrison, AMPA glutamate receptors and their flip and flop mRNAs in human hippocampus, NeuroReport, 5, pp. 1325-1328, (1994)

[9]

Garcia-Ladona, Palacios, Probst, Wieser, Mengod, Excitatory amino acid AMPA receptor mRNA localization in several regions of normal and neurological disease affected human brains. An in situ hybridization histochemistry study, Mol. Brain Res., 21, pp. 75-84, (1994)

[10]

Geddes, Cahan, Cooper, Kim, Choi, Cotman, Altered distribution of excitatory amino acid receptors in temporal lobe epilepsy, Exp. Neurol., 108, pp. 214-220, (1990)

← 1 2 3 4 →