DNA BIOSYNTHESIS IN MITOCHONDRIA - DIFFERENTIAL INHIBITION OF MITOCHONDRIAL AND NUCLEAR DNA POLYMERASES BY MUTAGENIC DYES ETHIDIUM BROMIDE AND ACRIFLAVIN

被引：133

作者：

MEYER, RR

SIMPSON, MV

机构：

[1] Department of Biological Sciences, State University of New York, Stony Brook, Long Island

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 34卷 / 02期

关键词：

D O I：

10.1016/0006-291X(69)90637-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The DNA-intercalating dyes ethidium bromide and acriflavin strongly inhibit the enzymatic synthesis of DNA catalyzed by rat liver mitochondrial DNA polymerase. When compared with the nuclear polymerase, the mitochondrial enzyme is much more sensitive to these dyes. These observations are in accord with the well known effects of these dyes in producing cytoplasmic (mitochondrial) but not nuclear mutations in yeast and trypanosomes. A possible mechanism for the mutagenic effect of the dyes is discussed. © 1969.

引用

页码：238 / &

共 23 条

[1]

BOLLUM FJ, 1959, J BIOL CHEM, V234, P2733

[2] STUDY OF THE CONDITIONS AND MECHANISM OF THE DIPHENYLAMINE REACTION FOR THE COLORIMETRIC ESTIMATION OF DEOXYRIBONUCLEIC ACID [J].