ANTISERUM TO A NOVEL PEPTIDE SEQUENCE REACTS SELECTIVELY WITH EPITHELIAL SUBPOPULATIONS

被引：2

作者：

CLARK, RK ^{[1
]}

LEE, EV ^{[1
]}

LOCASTRO, S ^{[1
]}

BHATNAGAR, P ^{[1
]}

ESSER, K ^{[1
]}

PRICHETT, W ^{[1
]}

DUNNINGTON, D ^{[1
]}

机构：

[1] SMITHKLINE BEECHAM PHARMACEUT,DEPT PEDTIDOMIMET RES,KING OF PRUSSIA,PA 19406

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1991年 / 39卷 / 10期

关键词：

EPITHELIUM; ANTISERUM; SQUAMOUS CELLS; DIFFERENTIATION;

D O I：

10.1177/39.10.1940312

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A synthetic peptide corresponding to a novel protein sequence isolated from bovine kidney was used to immunize rabbits. When applied to Western blots of bovine kidney extracts, antiserum to this peptide recognizes proteins with molecular weights of 23 and 18 KD. Immunohistochemical examination of a variety of bovine and rat tissues with this antiserum revealed a unique distribution of immunoreactivity with the intermediate layers of a variety of stratified epithelia, in addition to renal glomeruli. The pattern of reactivity differed from previously described epithelial markers such as cytokeratins. These results indicate that this antiserum may be useful as a tool for the identification of cells of the intermediate layer of stratified epithelia and, as such, may aid in the study of this differentiating/proliferating tissue compartment.

引用

页码：1409 / 1413

页数：5

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