VEGETATIVE LAMBDA DNA .4. FRACTIONATION OF REPLICATING LAMBDA DNA ON BENZOYLATED-NAPHTHOYLATED DEAE CELLULOSE

被引：102

作者：

KIGER, JA

SINSHEIMER, RL

机构：

[1] Division of Biology, California Institute of Technology Pasadena, CA

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1969年 / 40卷 / 03期

关键词：

D O I：

10.1016/0022-2836(69)90166-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fractionation on a column of benzoylated-naphthoylated DEAE cellulose has been used successfully to separate pulse-labeled, replicating bacteriophage lambda DNA from completed phage DNA or completed ring DNA molecules. Sedimentation analysis of the heterogeneous replicating DNA in neutral and alkaline gradients indicates the presence of closed single-strand rings and of linear DNA strands ranging in length from smaller than to not quite twice that of the viral DNA strands. A model of DNA replication is presented which can, by the timely interaction of specific nuclease or polynucleotide ligase enzymes, account for the various DNA forms encountered. © 1969.

引用

页码：467 / +

页数：1

共 48 条

[1] ANATOMY OF T5 BACTERIOPHAGE DNA MOLECULE [J].