INVITRO SYNTHESIS AND PROCESSING OF WHEAT ALPHA-AMYLASE - TRANSLATION OF GIBBERELLIC ACID-INDUCED WHEAT ALEURONE LAYER RNA BY WHEAT-GERM AND XENOPUS-LAEVIS OOCYTE SYSTEMS

被引：22

作者：

BOSTON, RS ^{[1
]}

MILLER, TJ ^{[1
]}

MERTZ, JE ^{[1
]}

BURGESS, RR ^{[1
]}

机构：

[1] UNIV WISCONSIN, MCARDLE LAB CANC RES, MADISON, WI 53706 USA

来源：

PLANT PHYSIOLOGY | 1982年 / 69卷 / 01期

关键词：

D O I：

10.1104/pp.69.1.150

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A 41,500-dalton protein was made which was identified as .alpha.-amylase by immunoprecipitation with rabbit anti-.alpha.-amylase antiserum raised against the purified wheat protein and by its co-migration with authentic .alpha.-amylase on sodium dodecyl sulfate polyacrylamide gels. Synthesis of .alpha.-amylase was dependent upon injection of RNA extract from GA-induced aleurone layers from wheat. The amount of .alpha.-amylase produced was proportional to the amount of RNA injected and reached a plateau within 4 h after injection. When the same RNA was translated in a wheat germ cell-free translation system, a 43,000-dalton protein was produced. Addition of dog pancreas microsomal membranes to the wheat germ translation system resulted in processing of the .alpha.-amylase protein to a form which co-migrated with authentic .alpha.-amylase purified from malted wheat and with the protein synthesized in oocytes.

引用

页码：150 / 154

页数：5

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