LYSOPHOSPHATIDIC ACID INDUCES A PERTUSSIS TOXIN-SENSITIVE CA2+-ACTIVATED CL- CURRENT IN XENOPUS-LAEVIS OOCYTES

被引:66
作者
DURIEUX, ME
SALAFRANCA, MN
LYNCH, KR
MOORMAN, JR
机构
[1] UNIV VIRGINIA,HLTH SCI CTR,DEPT PHYSIOL,CHARLOTTESVILLE,VA 22908
[2] UNIV VIRGINIA,HLTH SCI CTR,DEPT MED CARDIOL,CHARLOTTESVILLE,VA 22908
[3] UNIV VIRGINIA,HLTH SCI CTR,DEPT PHARMACOL & BIOCHEM,CHARLOTTESVILLE,VA 22908
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1992年 / 263卷 / 04期
关键词
G-PROTEIN-COUPLED RECEPTORS; CHLORIDE CHANNELS; SURAMIN; PLATELET-ACTIVATING FACTOR;
D O I
10.1152/ajpcell.1992.263.4.C896
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Lysophosphatidic acid (LPA) induces a Ca2+-activated Cl- current in defolliculated Xenopus laevis oocytes. The response appears mediated by a specific membrane receptor, because no current is induced when related compounds [phosphatidic acid (PA), lysophosphatidylcholine (LPC), and lysophosphatidylserine (LPS)] are applied extracellularly or when LPA is injected intracellularly. Incubation in pertussis toxin prevents the response. The response is mediated by a Ca2+-activated Cl- current because 1) it is abolished by intracellular ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA; 5 mM) but not affected by changes in extracellular Ca2+ concentration and 2) the reversal potential becomes more positive at lower Cl- concentrations. Suramin (2 mM) blocks the LPA-induced current, but PA, LPS, LPC, and the platelet-activating factor antagonist WEB-2086 do not. The response is dose dependent for LPA concentrations from 10(-8) to 10(-3) M. Incubation of oocytes in LPA does not induce germinal vesicle breakdown. These findings suggest that this novel oocyte response to LPA is mediated by a specific membrane receptor linked to a pertussis toxin-sensitive G protein.
引用
收藏
页码:C896 / C900
页数:5
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