REGULATION OF GLUTATHIONE S-TRANSFERASES OF ZEA-MAYS IN PLANTS AND CELL-CULTURES

An antiserum to glutathione S-transferase (EC 2.5.1.18) from maize (Zae mays L.) responsible for herbicide detoxification has been raised in rabbit. The antiserum was specific to the Mr 26000 subunit of the enzyme from maize seedlings and suspension-cultured cells, and recognized the isoenzymes active toward both atrazine and metolachlor. When plants were treated for 24 h with the herbicide antidote N,N-diallyl-2-2-dichloroacetamide (DDCA), enzyme activities toward metolachlor were doubled in the roots and this was associated with a 70% increase in immunodetectable protein. Translation of polysomal RNA in vitro showed that the increase in the transferase in root tissue was brought about by a ninefold increase in mRNA activity encoding the enzyme. Treatment of suspension-cultured cells with cinamic acid, metolachlor and DDCA raised enzyme activities but did not increase synthesis of glutathione S-transferase. In cultured maize cells, enzyme synthesis was maximal in mid-logarithmic phase, coinciding with the highest levels of enzyme activity. When callus cultures were established from the shoots of a mazie line know to conjugate chloro-s-triazines, enzyme activity towards atrazine was lost during primary dedifferentitaion. However, levels of total immodetectable enzyme and activity toward metolachlor were increased in cultured cells compared with the parent shoot tissue.