MOLECULAR-CLONING OF COPPER RESISTANCE GENES FROM PSEUDOMONAS-SYRINGAE PV TOMATO

被引：115

作者：

BENDER, CL ^{[1
]}

COOKSEY, DA ^{[1
]}

机构：

[1] UNIV CALIF RIVERSIDE, DEPT PLANT PATHOL, RIVERSIDE, CA 92521 USA

来源：

JOURNAL OF BACTERIOLOGY | 1987年 / 169卷 / 02期

关键词：

D O I：

10.1128/jb.169.2.470-474.1987

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A cosmid library of copper-resistant (Cur) Pseudomonas syringae pv. tomato PT23 plasmid DNA was constructed and mobilized into the copper-sensitive recipient P. syringae pv. syringae PS61. One resultant cosmid clone, pCOP1 (46 kilobases) conferred copper resistance. The PT23 Cur gene(s) was located on pCOP1 by subcloning PstI restriction endonuclease fragments of pCOP1 in the broad-host-range vector pRK404. A subclone containing a 4.4-kilobases PstI fragment conferred Cur on PS61. The Cur gene(s) was further located by insertional inactivation with Tn5. A subcloned fragment internal to the Cur determinant on pCOP2 was probed to plasmid and chromosomal DNA of four copper-resistant and three copper-sensitive strains of P. syringae p.v.tomato. The probe hybridized to plasmids in resistant strains, but showed no detectable homology to copper-sensitive strains.

引用

页码：470 / 474

页数：5

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