ENVELOPE GLYCOPROTEINS OF HIV-1, HIV-2, AND SIV PURIFIED WITH GALANTHUS-NIVALIS AGGLUTININ INDUCE STRONG IMMUNE-RESPONSES

被引：72

作者：

GILLJAM, G

机构：

[1] Department of Virology, National Bacteriological Laboratory

来源：

AIDS RESEARCH AND HUMAN RETROVIRUSES | 1993年 / 9卷 / 05期

关键词：

D O I：

10.1089/aid.1993.9.431

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Lectin affinity chromatography was used to purify in a single step the envelope glycoproteins of HIV-1, HIV-2, and SIV. Envelope glycoproteins carry the major determinants essential for protection by the humoral immune response. The purification of these proteins has previously been a laborious procedure. The glycoproteins were purified by a one-step procedure to a high level of purity by using Galanthus nivalis agglutinin (GNA). The purified glycoprotein had CD4-binding and antigenic reactivities. Strong immune responses to envelope proteins and peptides were seen in mice and primates after immunization with these preparations.

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页码：431 / 438

页数：8

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