DIRECT VISUALIZATION AND QUANTIFICATION OF CELLULAR CYTOTOXICITY USING 2 COLOR FLUORESCENCE

被引:60
作者
KROESEN, BJ
MESANDER, G
TERHAAR, JG
THE, TH
DELEIJ, L
机构
[1] Department of Clinical Immunology, Internal Medicine, University Hospital Groningen, 9713 EZ Groningen
关键词
MEMBRANE FLUORESCENCE; CELLULAR CYTOTOXICITY; NATURAL KILLER CELL; LYMPHOKINE ACTIVATED KILLER CELL; ACTIVATED T-CELL;
D O I
10.1016/0022-1759(92)90009-I
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A fluorescence method is described for the evaluation of cell death induced by cellular cytolytic activity. A green fluorescent membrane dye, D275, was used to label various target cell lines and propidium iodide (PI) uptake was used to assay cell death. Natural killer (NK), lymphokine activated killer (LAK) as well as targeted T cell mediated cellular cytotoxicity were quantified using the fluorescence method and compared to results obtained with the chromium-51 (Cr-51) release assay. A good correlation was found after an assay period of 4-8 h indicating that the fluorescence method is a reliable alternative to the Cr-51 release assay.
引用
收藏
页码:47 / 54
页数:8
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