MEMBRANE-TRANSPORT OF L-TRIIODTHYRONINE BY HUMAN RED-CELL GHOSTS

被引：55

作者：

HOLM, AC ^{[1
]}

JACQUEMIN, C ^{[1
]}

机构：

[1] FAC SCI REIMS,BIOCHEM LAB,F-51062 REIMS,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 89卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91877-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

L-T3 transport has been investigated in human red cell ghosts. Determination of initial T3 uptake revealed two separate saturable uptake systems, one with a Km of 1.6 × 10-8M, the other with a Km of 3.3 × 10-6M. Binding experiments resulted in two dissociation constants, 1.4 × 10-7M.and 2.6 × 10-6M. Uptake was dependent on the ghost volume, indicating an intravesicular location of T3. The T3 was concentrated 6 times by the ghosts. Ouabain reduced the uptake by the low Km system, but was without effect on the high Km system. Thus evidence is provided both of binding of T3 to the ghost membrane and of its uphill transport across the membrane. © 1979.

引用

页码：1006 / 1017

页数：12

共 22 条

[1] SELECTIVE ALTERATION BY TRIIODOTHYRONINE OF AMINO ACID TRANSPORT IN EMBRYONIC BONE [J].