CELL-GROWTH EFFECTS OF EPSTEIN-BARR-VIRUS LEADER PROTEIN

被引：35

作者：

ALLAN, GJ ^{[1
]}

INMAN, GJ ^{[1
]}

PARKER, BD ^{[1
]}

ROWE, DT ^{[1
]}

FARRELL, PJ ^{[1
]}

机构：

[1] ST MARYS HOSP,SCH MED,LUDWIG INST CANC RES,NORFOLK PL,LONDON W2 1PG,ENGLAND

来源：

JOURNAL OF GENERAL VIROLOGY | 1992年 / 73卷

关键词：

D O I：

10.1099/0022-1317-73-6-1547

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

B lymphoblastoid cell lines immortalized with P3HR1/633 Epstein-Barr virus (EBV), which has a deletion in the EBV nuclear antigen leader protein (EBNA-LP) gene, were transfected with a vector expressing wild-type EBNA-LP. The EBNA-LP transfectants grew out faster under G418 selection than control cells but expression of EBNA-LP made no significant difference to growth rate or saturation density of the resulting established cell lines. When the cells expressing EBNA-LP were allowed to grow to saturation and then diluted in fresh medium they underwent DNA synthesis more rapidly than control cultures.

引用

页码：1547 / 1551

页数：5

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