FORMATION OF N-FORMYL-METHIONYL-PUROMYCIN BY INTACT CELLS OF 4 DIFFERENT BACTERIA AND A BLUE-GREEN ALGA

被引：18

作者：

BACHMAYER, H

KREIL, G

机构：

[1] Institute of Molecular Biology, Austrian Academy of Sciences, 1090 Vienna

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1968年 / 169卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0005-2787(68)90011-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intact procaryotic cells, incubated in the presence of puromycin and labelled methionine, synthesize N-formyl-methionyl-puromycin. This has been demonstrated with Escherichia coli, Bacillus subtilis, Micrococcus lysodeikticus, Pseudomonas aeruginosa and with the blue-green alga Anacystis nidulans. The yield of N-formyl-methionyl-puromycin increases with the concentration of puromycin in the medium and is lowered by choramphenicol. According to present evidence this represents a simple in vivo test for chain initiators of protein biosynthesis. Possible applications of this approach are discussed. © 1968.

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页码：95 / +

页数：1

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