NEUTRALIZATION OF CHLAMYDIA-TRACHOMATIS - KINETICS AND STOICHIOMETRY

被引:33
作者
PEELING, RW [1 ]
BRUNHAM, RC [1 ]
机构
[1] UNIV MANITOBA, DEPT MED MICROBIOL, WINNIPEG R3E 0W3, MANITOBA, CANADA
关键词
D O I
10.1128/IAI.59.8.2624-2630.1991
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Monoclonal antibodies to the major outer membrane protein of Chlamydia trachomatis were used to neutralize C. trachomatis infectivity in HeLa 229 cells and to determine the kinetics and stoichiometry of the reaction. In vitro neutralization of C. trachomatis infectivity proceeded as a first-order reaction and required an activation energy of approximately 20 kcal/mol (ca. 84 kJ/mol). The rate of neutralization was linear with respect to antibody concentration and reaction temperature. The efficiency of neutralization decreased exponentially as the ratio of noninfective to infective chlamydiae increased in the antigen preparation. The neutralization assay was also significantly affected by reaction parameters such as the reaction volume and the duration of incubation. Stoichiometric calculations showed that an average ratio of 10(3) and 10(4) immunoglobulin molecules per chlamydial particle was required to yield 50% neutralization by monoclonal antibodies specifying serovar-specific and species-specific epitopes, respectively. The implications of these findings for vaccine design and for the role of the major outer membrane protein in the pathogenesis of chlamydial infections are discussed.
引用
收藏
页码:2624 / 2630
页数:7
相关论文
共 27 条
[1]   PICORNAVIRUSES ARE NO LONGER BLACK-BOXES [J].
BALTIMORE, D .
SCIENCE, 1985, 229 (4720) :1366-1367
[2]  
BANKS J, 1970, Infection and Immunity, V2, P443
[3]  
BARENFAN.J, 1974, J IMMUNOL, V113, P1607
[4]   NEUTRALIZATION OF TRIC ORGANISMS BY ANTIBODY - ENHANCEMENT BY ANTISERA PREPARED AGAINST IMMUNOGLOBULINS [J].
BLYTH, WA ;
TAVERNE, J .
JOURNAL OF HYGIENE, 1974, 72 (01) :129-134
[5]  
BLYTH WA, 1962, BRIT J EXP PATHOL, V43, P340
[6]   POSTABORTAL CHLAMYDIA-TRACHOMATIS SALPINGITIS - CORRELATING RISK WITH ANTIGEN-SPECIFIC SEROLOGICAL RESPONSES AND WITH NEUTRALIZATION [J].
BRUNHAM, RC ;
PEELING, R ;
MACLEAN, I ;
MCDOWELL, J ;
PERSSON, K ;
OSSER, S .
JOURNAL OF INFECTIOUS DISEASES, 1987, 155 (04) :749-755
[7]   NEUTRALIZATION OF CHLAMYDIA-TRACHOMATIS INFECTIVITY WITH ANTIBODIES TO THE MAJOR OUTER-MEMBRANE PROTEIN [J].
CALDWELL, HD ;
PERRY, LJ .
INFECTION AND IMMUNITY, 1982, 38 (02) :745-754
[8]   LOCALIZATION OF DISTINCT SURFACE-ANTIGENS ON CHLAMYDIA-TRACHOMATIS HAR-13 BY IMMUNE ELECTRON-MICROSCOPY WITH MONOCLONAL-ANTIBODIES [J].
CLARK, RB ;
NACHAMKIN, I ;
SCHATZKI, PF ;
DALTON, HP .
INFECTION AND IMMUNITY, 1982, 38 (03) :1273-1278
[9]   A STUDY OF THE BASIC ASPECTS OF NEUTRALIZATION OF 2 ANIMAL VIRUSES, WESTERN EQUINE ENCEPHALITIS VIRUS AND POLIOMYELITIS VIRUS [J].
DULBECCO, R ;
VOGT, M ;
STRICKLAND, AGR .
VIROLOGY, 1956, 2 (02) :162-205
[10]   THE TITRATION OF TRACHOMA AND INCLUSION BLENNORRHOEA VIRUSES IN CELL CULTURES [J].
FURNESS, G ;
GRAHAM, DM ;
REEVE, P .
JOURNAL OF GENERAL MICROBIOLOGY, 1960, 23 (03) :613-619