IDENTIFICATION OF A LATENT MAP KINASE KINASE KINASE IN PC12 CELLS AS B-RAF

被引：35

作者：

TRAVERSE, S

COHEN, P

机构：

[1] MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee

来源：

FEBS LETTERS | 1994年 / 350卷 / 01期

关键词：

MAP KINASE; PC12; CELL; RAF; NERVE GROWTH FACTOR; EPIDERMAL GROWTH FACTOR;

D O I：

10.1016/0014-5793(94)00723-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A latent MAP kinase kinase kinase activity previously detected after chromatography of PC12 cell extracts on Mono Q [(1992) FEBS Lett. 314, 461-465] has been identified as B-Raf by immunoblotting and immunoprecipitation experiments and by its specific activation with B-Raf antibodies. B-Raf is phosphorylated after stimulation of PC12 cells with nerve growth factor or epidermal growth factor, but this is not accompanied by activation of the Mono Q-purified enzyme whether assayed in the absence or presence of GTP-Ras.

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页码：13 / 18

页数：6

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