EFFECTS OF HERBIMYCIN-A AND VARIOUS SH-REAGENTS ON P60V-SRC KINASE-ACTIVITY INVITRO

被引：49

作者：

FUKAZAWA, H

MIZUNO, S

UEHARA, Y

机构：

[1] Department of Antibiotics, National Institute of Health, Shinagawa-ku, Tokyo, 141

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 173卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(05)81053-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Herbimycin A is an antibiotic which reverses transformation caused by src family oncogenes. It inactivates p60v-src in vitro, possibly by binding to reactive SH-group(s) of the kinase. We examined effects of various SH-reagents on p60v-src and observed that N-[p-(2-benzimidazolyl)phenyl]maleimide (BIPM) or N-(9-acridinyl)maleimide (NAM) were potent inactivators of the kinase, whereas N-ethylmaleimide (NEM) required high concentrations, and iodoacetamide was totally ineffective in reducing the kinase activity. Pretreatment of p60v-src immune-complex with NEM and iodoacetamide, however, protected the kinase from inactivation by herbimycin A, BIPM, and NAM. The results suggest that SH-group(s) to which herbimycin A binds is not essential for the kinase activity, but is positioned in the vicinity of the active center. © 1990 Academic Press, Inc.

引用

页码：276 / 282

页数：7

共 6 条

[1]

JOVE R, 1987, ANNU REV CELL BIOL, V3, P31, DOI 10.1146/annurev.cellbio.3.1.31

[2] FLUORESCENCE AND STRUCTURES OF PROTEINS AS MEASURED BY INCORPORATION OF FLUOROPHORE .4. SYNTHESIS AND FLUORESCENCE CHARACTERISTICS OF N-PARA-(2-BENZIMIDAZOLYL)PHENYL) MALEIMIDE [J].