A NEW VARIANT OF GLYCOPROTEIN CD44 CONFERS METASTATIC POTENTIAL TO RAT CARCINOMA-CELLS

被引：1630

作者：

GUNTHERT, U

HOFMANN, M

RUDY, W

REBER, S

ZOLLER, M

HAUSSMANN, I

MATZKU, S

WENZEL, A

PONTA, H

HERRLICH, P

机构：

[1] GERMAN CANC RES CTR,INST RADIOL & PATHOPHYSIOL,W-6900 HEIDELBERG 1,GERMANY

[2] KERNFORSCHUNGSZENTRUM KARLSRUHE GMBH,INST GENET & TOXIKOL,W-7500 KARLSRUHE 1,GERMANY

来源：

CELL | 1991年 / 65卷 / 01期

关键词：

D O I：

10.1016/0092-8674(91)90403-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using a monoclonal antibody (MAb1.1ASML) raised against a surface glycoprotein of the metastasizing rat pancreatic carcinoma cell line BSp73ASML, cDNA clones have been isolated that encode glycoproteins with partial homology to CD44, a presumed adhesion molecule. In one of the clones, pMeta-1, the epitope marks an additional extracellular domain of 162 amino acids inserted into the rat CD44 protein between amino acid positions 223 and 247 (by analogy to human and murine CD44). The new variants are expressed only in the metastasizing cell lines of two rat tumors, the pancreatic carcinoma BSp73 and the mammary adenocarcinoma 13762NF; they are not expressed in the nonmetastasizing tumor cell lines nor in most normal rat tissues. Overexpression of pMeta-1 in the nonmetastasizing BSp73AS cells suffices to establish full metastatic behavior.

引用

页码：13 / 24

页数：12

共 65 条

[1] CD44 IS THE PRINCIPAL CELL-SURFACE RECEPTOR FOR HYALURONATE [J].

ARUFFO, A ;

STAMENKOVIC, I ;

MELNICK, M ;

UNDERHILL, CB ;

SEED, B .

CELL, 1990, 61 (07) :1303-1313

[2] TRANSCRIPTION TERMINATION AND 3' PROCESSING - THE END IS IN SITE [J].

BIRNSTIEL, ML ;

BUSSLINGER, M ;

STRUB, K .

CELL, 1985, 41 (02) :349-359

[3]

BOYLAN JF, 1990, ANTICANCER RES, V10, P717

[4]

CARR I, 1981, Invasion and Metastasis, V1, P34

[5]

CARTER WG, 1988, J BIOL CHEM, V263, P4193

[6] PREDICTION OF PROTEIN CONFORMATION [J].

CHOU, PY ;

FASMAN, GD .

BIOCHEMISTRY, 1974, 13 (02) :222-245

[7]

COLLARD JG, 1987, CANCER RES, V47, P754

[8]

DEAR TN, 1989, CANCER RES, V49, P5323

[9]

DENNING SM, 1990, J IMMUNOL, V144, P7

[10] EXPRESSION OF H-RAS CORRELATES WITH METASTATIC POTENTIAL - EVIDENCE FOR DIRECT REGULATION OF THE METASTATIC PHENOTYPE IN 10T1/2 AND NIH 3T3 CELLS [J].

EGAN, SE ;

MCCLARTY, GA ;

JAROLIM, L ;

WRIGHT, JA ;

SPIRO, I ;

HAGER, G ;

GREENBERG, AH .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (02) :830-837

← 1 2 3 4 5 6 7 →