SIMPLE AND RELIABLE TECHNIQUE FOR SEPARATING THE ANDROGEN RECEPTOR FROM TESTOSTERONE-BINDING GLOBULIN IN HUMAN TISSUES

被引：12

作者：

MOWSZOWICZ, I

WRIGHT, F

机构：

[1] Service de Biochimie Médicale, Faculté de Médeccine Pitie-Salpetriere, 75634 Paris Cedex 13, 91, Boulevard de l'Hopital

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 92卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90639-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ammonium sulfate precipitation, followed by Sepharose 4B column chromatography is used to separate the androgen receptor from contaminating testosterone-binding globulin in human foreskin cytosol. The binding specificity is checked by dihydrotestosterone and cyproterone acetate displacement. In addition, after the column, the tubes corresponding to the peak of receptor can be pooled and used in an assay. This simple and reliable technique should allow the study of the androgen receptor in human target tissues. © 1979.

引用

页码：164 / 169

页数：6

共 17 条

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