DICTYOSTELIUM MUTANTS LACKING THE CYTOSKELETAL PROTEIN CORONIN ARE DEFECTIVE IN CYTOKINESIS AND CELL MOTILITY

被引：209

作者：

DEHOSTOS, EL ^{[1
]}

REHFUESS, C ^{[1
]}

BRADTKE, B ^{[1
]}

WADDELL, DR ^{[1
]}

ALBRECHT, R ^{[1
]}

MURPHY, J ^{[1
]}

GERISCH, G ^{[1
]}

机构：

[1] MAX PLANCK INST BIOCHEM,W-8033 MARTINSRIED,GERMANY

来源：

JOURNAL OF CELL BIOLOGY | 1993年 / 120卷 / 01期

关键词：

D O I：

10.1083/jcb.120.1.163

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Coronin is an actin-binding protein in Dictyostelium discoideum that is enriched at the leading edge of the cells and in projections of the cell surface called crowns. The polypeptide sequence of coronin is distinguished by its similarities to the beta-subunits of trimeric G proteins (E. L. de Hostos, B. Bradtke, F. Lottspeich, R. Guggenheim, and G. Gerisch. 1991. EMBO (Eur Mol. Biol. Organ.) J. 10:4097-4104). To elucidate the in vivo function of coronin, null mutants have been generated by gene replacement. The mutant cells lacking coronin grow and migrate more slowly than wild-type cells. When these cor- cells grow in liquid medium they become multinucleate, indicating a role of coronin in cytokinesis. To explore this role, coronin has been localized in mitotic wild-type cells by immunofluorescence labeling. During separation of the daughter cells, coronin is strongly accumulated at their distal portions including the leading edges. This contrasts with the localization of myosin II in the cleavage furrow and suggests that coronin functions independently of the conventional myosin in facilitating cytokinesis.

引用

页码：163 / 173

页数：11

共 46 条

[1] A DICTYOSTELIUM MUTANT DEFICIENT IN SEVERIN, AN F-ACTIN FRAGMENTING PROTEIN, SHOWS NORMAL MOTILITY AND CHEMOTAXIS [J].