PREPARATION AND PROPERTIES OF ISOLATED ALPHA AND BETA CHAINS OF HUMAN HEMOGLOBIN IN FERRI STATE - INVESTIGATION OF OXIDATION-REDUCTION EQUILIBRIA

被引:63
作者
BANERJEE, R
CASSOLY, R
机构
[1] Institut de Biologie Physico-Chimique, Paris 5, 13, rue Pierre et Marie Curie
关键词
D O I
10.1016/0022-2836(69)90047-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The ferric derivatives of isolated α and β chains of human hemoglobin, known to be rapidly denatured under ordinary conditions, have been prepared in stable form through the choice of an appropriate solvent (1 m-glycine). Preparations in 1 m 1-glycine have been used to study the oxidation-reduction equilibria of the chains. The redox equilibria of the isolated chains are different in the pH range studied (pH 6 to 8), the electron affinity of the β chain being higher (Em7 = + 0.113v) than that of the α chain (Em7 = + 0.052 v); furthermore, the β chains possess an additional oxidation-linked ionization (pK′ ~ 7) not revealed in chain α. The results are discussed in the context of human hemoglobin; they lend support to the idea of intrinsic non-equivalence of sites in the oxidation-reduction equilibrium of the latter. © 1969.
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页码:337 / &
相关论文
共 19 条
[1]  
ANTONINI E, 1964, J BIOL CHEM, V239, P907
[2]   SUBUNIT EXCHANGE AND LIGAND BINDING - A NEW HYPOTHESIS FOR MECHANISM OF OXYGENATION OF HEMOGLOBIN [J].
BENESCH, RE ;
BENESCH, R ;
MACDUFF, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1965, 54 (02) :535-&
[3]  
BRAUNITZER G, 1958, Z PHYSIOL CHEM, V312, P72
[4]  
BRUNORI M, 1964, J BIOL CHEM, V239, P2340
[5]  
BRUNORI M, 1965, J BIOL CHEM, V240, P3317
[6]  
BUCCI E, 1965, J BIOL CHEM, V240, pO551
[7]  
Clark W.M., 1960, OXIDATION REDUCTION
[8]   The effect of methemoglobin on the equilibrium between oxygen and hemoglobin [J].
Darling, RC ;
Roughton, FJW .
AMERICAN JOURNAL OF PHYSIOLOGY, 1942, 137 (01) :0056-0068
[9]  
DERENZO EC, 1967, J BIOL CHEM, V242, P4850
[10]  
DRABKIN DL, 1946, J BIOL CHEM, V164, P703