PHYSICAL MAPPING OF A PLASMID FROM BACILLUS-MEGATERIUM BY RESTRICTION ENDONUCLEASE CLEAVAGE

被引：15

作者：

CARLTON, BC

BROWN, BJ

机构：

[1] UNIV GEORGIA,DEPT BIOCHEM,ATHENS,GA 30602

[2] UNIV GEORGIA,DEPT MICROBIOL,ATHENS,GA 30602

来源：

PLASMID | 1979年 / 2卷 / 01期

关键词：

D O I：

10.1016/0147-619X(79)90006-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A 4-Mdalton plasmid from Bacillus megaterium strain 216 has been physically mapped by restriction endonuclease digestion. A combination of single and double digests with seven restriction enzymes, together with a terminal labeling procedure, has produced a physical map containing 21 apparently unique cleavage sites. The data are most consistent with the view that this plasmid does not contain extensive variability in sequence. © 1979.

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页码：59 / 68

页数：10

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