ADENOVIRUS TYPE-5 PRECURSOR TERMINAL PROTEIN-EXPRESSING 293 AND HELA-CELL LINES

被引:34
作者
SCHAACK, J
GUO, XL
HO, WYW
KARLOK, M
CHEN, CY
ORNELLES, D
机构
[1] UNIV COLORADO,HLTH SCI CTR,PROGRAM MOLEC BIOL,DENVER,CO 80262
[2] UNIV COLORADO,HLTH SCI CTR,CTR CANC,DENVER,CO 80262
[3] STANFORD UNIV,DEPT IMMUNOL,STANFORD,CA 94305
[4] WAKE FOREST UNIV,BOWMAN GRAY SCH MED,DEPT MICROBIOL & IMMUNOL,WINSTON SALEM,NC 27157
关键词
D O I
10.1128/JVI.69.7.4079-4085.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
HeLa and 293 cell lines that express biologically active adenovirus type 5 precursor terminal protein (pTP) have been made. The amount of pTP synthesized in these cell lines ranges from barely detectable to greater than that observed in cells infected with the wild-type virus. The pTP-expressing cell lines permit the growth of a temperature-sensitive terminal protein mutant virus sub100r at the nonpermissive temperature. A higher percentage of the stably transfected 293 cell lines expressed terminal protein, and generally at considerably higher levels, than did the HeLa cell lines. While 293 cells appeared to tolerate pTP better than did HeLa cells, high-level pTP expression in 293 cells led to a significantly reduced growth rate. The 293-pTP cell lines produce infectious virus after transfection with purified viral DNA and form plaques when overlaid with Noble agar after infection at low multiplicity. These cell lines offer promise for the production of adenoviruses lacking pTP expression and therefore completely defective for replication.
引用
收藏
页码:4079 / 4085
页数:7
相关论文
共 34 条
[1]   THE TERMINAL REGIONS OF ADENOVIRUS AND MINUTE VIRUS OF MICE DNAS ARE PREFERENTIALLY ASSOCIATED WITH THE NUCLEAR MATRIX IN INFECTED-CELLS [J].
BODNAR, JW ;
HANSON, PI ;
POLVINOBODNAR, M ;
ZEMPSKY, W ;
WARD, DC .
JOURNAL OF VIROLOGY, 1989, 63 (10) :4344-4353
[2]   CONSTRUCTION, CHARACTERIZATION, AND UTILIZATION OF CELL-LINES WHICH INDUCIBLY EXPRESS THE ADENOVIRUS DNA-BINDING PROTEIN [J].
BROUGH, DE ;
CLEGHON, V ;
KLESSIG, DF .
VIROLOGY, 1992, 190 (02) :624-634
[3]   CELL CYCLE-ASSOCIATED CHANGE IN THE EXPRESSION OF THE PROLIFERATION-SENSITIVE AND HEAT-SHOCK PROTEIN HSX70 (IEF14) - INCREASED SYNTHESIS DURING MITOSIS [J].
CELIS, JE ;
LAURIDSEN, JB ;
BASSE, B .
EXPERIMENTAL CELL RESEARCH, 1988, 177 (01) :176-185
[4]   ABLATION OF E2A IN RECOMBINANT ADENOVIRUSES IMPROVES TRANSGENE PERSISTENCE AND DECREASES INFLAMMATORY RESPONSE IN MOUSE-LIVER [J].
ENGELHARDT, JF ;
YE, XH ;
DORANZ, B ;
WILSON, JM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (13) :6196-6200
[5]   ADENOVIRUS PRECURSOR TO TERMINAL PROTEIN INTERACTS WITH THE NUCLEAR MATRIX INVIVO AND INVITRO [J].
FREDMAN, JN ;
ENGLER, JA .
JOURNAL OF VIROLOGY, 1993, 67 (06) :3384-3395
[6]   CODON INSERTION MUTANTS OF THE ADENOVIRUS TERMINAL PROTEIN [J].
FREIMUTH, PI ;
GINSBERG, HS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (20) :7816-7820
[7]   ROLE OF EARLY GENES IN PATHOGENESIS OF ADENOVIRUS PNEUMONIA [J].
GINSBERG, HS ;
HORSWOOD, RL ;
CHANOCK, RM ;
PRINCE, GA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (16) :6191-6195
[8]   A MOUSE MODEL FOR INVESTIGATING THE MOLECULAR PATHOGENESIS OF ADENOVIRUS PNEUMONIA [J].
GINSBERG, HS ;
MOLDAWER, LL ;
SEHGAL, PB ;
REDINGTON, M ;
KILIAN, PL ;
CHANOCK, RM ;
PRINCE, GA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (05) :1651-1655
[9]   TIGHT CONTROL OF GENE-EXPRESSION IN MAMMALIAN-CELLS BY TETRACYCLINE-RESPONSIVE PROMOTERS [J].
GOSSEN, M ;
BUJARD, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (12) :5547-5551
[10]   CHARACTERISTICS OF A HUMAN CELL LINE TRANSFORMED BY DNA FROM HUMAN ADENOVIRUS TYPE-5 [J].
GRAHAM, FL ;
SMILEY, J ;
RUSSELL, WC ;
NAIRN, R .
JOURNAL OF GENERAL VIROLOGY, 1977, 36 (JUL) :59-72