THE ACTIVE MONOMERIC FORM OF MACROPHAGE INFLAMMATORY PROTEIN-1-ALPHA INTERACTS WITH HIGH-AFFINITY AND LOW-AFFINITY CLASSES OF RECEPTORS ON HUMAN HEMATOPOIETIC-CELLS

被引:29
作者
AVALOS, BR [1 ]
BARTYNSKI, KJ [1 ]
ELDER, PJ [1 ]
KOTUR, MS [1 ]
BURTON, WG [1 ]
WILKIE, NM [1 ]
机构
[1] OHIO STATE UNIV HOSP,DEPT INTERNAL MED,DIV HEMATOL & ONCOL,DIV BONE MARROW TRANSPLANTAT,COLUMBUS,OH 43210
关键词
D O I
10.1182/blood.V84.6.1790.bloodjournal8461790
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Macrophage inflammatory protein-1 alpha (MIP-1 alpha) and its human homologue GOS19.1/LD78 are members of the C-C chemokine/intercrine family of secreted proteins. They have proinflammatory properties and also inhibit cell cycle progression of hematopoietic stem cells. Characterization of MIP-1 alpha receptor(s) has been confused because of its reported aggregation to inactive forms. Using a defined monomeric form of MIP-1 alpha that is biologically active for stem cell inhibition and induction of oxidative metabolism in polymor-phonuclear cells, we report the detection of high- and low-affinity receptor classes on human leukemic CD34(+) blast cells, promyelocytic cells, monocytes, peripheral blood neutrophils, and T cells. Both high- and low-affinity classes are expressed simultaneously in promyelocytes and neutrophils. The calculated kd for high-affinity receptors correlates with the concentrations of MIP-1 alpha required to induce a biologic effect on stem cells and neutrophils. Cross-linking studies show that MIP-1 alpha associates with two cell surface proteins with apparent molecular masses of 92 kD and 52 kD. Direct competition binding studies combined with studies on the inhibition of stem cells show that human and murine MIP-1 alpha have different receptor-binding and biologic properties. (C) 1994 by The American Society of Hematology.
引用
收藏
页码:1790 / 1801
页数:12
相关论文
共 52 条
[1]   MACROPHAGE INFLAMMATORY PROTEIN-1-ALPHA ACTIVATES BASOPHILS AND MAST-CELLS [J].
ALAM, R ;
FORSYTHE, PA ;
STAFFORD, S ;
LETTBROWN, MA ;
GRANT, JA .
JOURNAL OF EXPERIMENTAL MEDICINE, 1992, 176 (03) :781-786
[2]   NEUTROPHIL-ACTIVATING PEPTIDE-1 INTERLEUKIN-8, A NOVEL CYTOKINE THAT ACTIVATES NEUTROPHILS [J].
BAGGIOLINI, M ;
WALZ, A ;
KUNKEL, SL .
JOURNAL OF CLINICAL INVESTIGATION, 1989, 84 (04) :1045-1049
[3]  
BASS DA, 1983, J IMMUNOL, V130, P1910
[4]  
BEUUTLER B, 1985, J EXP MED, V161, P1984
[5]   3 HUMAN HOMOLOGS OF A MURINE GENE ENCODING AN INHIBITOR OF STEM-CELL PROLIFERATION [J].
BLUM, S ;
FORSDYKE, RE ;
FORSDYKE, DR .
DNA AND CELL BIOLOGY, 1990, 9 (08) :589-602
[6]  
BROXMEYER HE, 1990, BLOOD, V76, P1110
[7]  
BROXMEYER HE, 1991, J IMMUNOL, V147, P2586
[8]   MEASUREMENT OF SPECIFIC RADIOACTIVITIES IN LABELED HORMONES BY SELF-DISPLACEMENT ANALYSIS [J].
CALVO, JC ;
RADICELLA, JP ;
CHARREAU, EH .
BIOCHEMICAL JOURNAL, 1983, 212 (02) :259-264
[9]  
Charles RS, 1989, J BIOL CHEM, V264, P2092
[10]   3-DIMENSIONAL STRUCTURE OF INTERLEUKIN-8 IN SOLUTION [J].
CLORE, GM ;
APPELLA, E ;
YAMADA, M ;
MATSUSHIMA, K ;
GRONENBORN, AM .
BIOCHEMISTRY, 1990, 29 (07) :1689-1696