SECRETAGOGUE-SPECIFIC EFFECTS OF INTERLEUKIN-1 ON GASTRIC-ACID SECRETION

Interleukin-1-beta (IL-1-beta) has recently been shown to reduce the severity of experimental gastroduodenal damage and to inhibit acid secretion in the pylorus-ligated rat. In the present study, the effects of IL-1-beta on pentagastrin-stimulated acid secretion were compared with those of two other cytokines, namely IL-1-alpha and tumor necrosis factor (TNF)alpha. Also, the effects of IL-1-beta on gastric acid secretion stimulated by bethanechol or histamine were assessed. Anesthetized rats were pretreated intravenously with one of the cytokines, at doses in the 0.1-5-mu-g/kg range, 30 min before starting an intravenous infusion of pentagastrin. TNF-alpha failed to significantly affect acid secretion, whereas IL-1-alpha and IL-1-beta exhibited significant inhibitory effects. For example, at a dose of 5-mu-g/kg, IL-1-alpha and IL-1-beta reduced acid secretion by 33 and 80%, respectively. The inhibitory effects of IL-1-beta on acid secretion could be completely inhibited by preincubation with an antibody directed against IL-1-beta but not by pretreatment with indomethacin (5 mg/kg sc) or by bilateral vagotomy. If acid secretion was stimulated by intravenous infusions of histamine or bethanechol, neither IL-1-beta nor TNF-alpha produced significant inhibitory effects. Inhibition of acid secretion by IL-1 was also observed when the IL-1 was administered subsequent to stimulation by pentagastrin administration. These results demonstrate that IL-1-beta is an extremely potent inhibitor of acid secretion stimulated by pentagastrin but not that stimulated by histamine or bethanechol, through a mechanism that is at least in part independent of the vagus nerve and of prostaglandin synthesis. IL-1-alpha is less potent as an inhibitor of gastric acid secretion, whereas TNF appears to be inactive. Because pentagastrin-stimulated acid secretion could be completely inhibited by a histamine H2-receptor antagonist (cimetidine) and because IL-1 had no effect on histamine-stimulated acid secretion, it is possible that IL-1 exerts its antisecretory actions by inhibiting pentagastrin-stimulated histamine release.