DEFECTIVE PHAGE FORMATION BY LYSOGENS OF INTEGRATION DEFICIENT PHAGE P22 MUTANTS

被引：78

作者：

SMITH, HO

机构：

[1] Department of Human Genetics, University of Michigan, Ann Arbor, MI

[2] the Lawrence D. Buhl Research Center for Human Genetics, University of Michigan, Ann Arbor, MI

来源：

VIROLOGY | 1968年 / 34卷 / 02期

关键词：

D O I：

10.1016/0042-6822(68)90231-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Integration deficient (L) mutants of phage P22 can be complemented with L+ phage to yield L mutant lysogens. Once established the L prophage is stable in the absence of L+ function. Ultraviolet or thermal induction of L lysogens leads to production of defective phage particles and low yields of infectious phage. The defective particles contain a normal amount of DNA but the DNA is mainly bacterial in origin and appears to derive from the pro C region adjacent to one end of the prophage. The defective lysates transduce pro C at high frequency relative to other bacterial markers. The representation of phage genes is strongly polar with markers distal to pro C being rarely present. It appears that L+ function is required not only for efficient integration of the prophage, but for normal recovery of the prophage genome following induction. © 1968.

引用

页码：203 / &

共 27 条

[1] CHROMOSOME TRANSFER IN BACTERIAL CONJUGATION [J].