THE COMET ASSAY - A COMPREHENSIVE REVIEW

被引：1366

作者：

FAIRBAIRN, DW

OLIVE, PL

ONEILL, KL

机构：

[1] BRIGHAM YOUNG UNIV, DEPT MICROBIOL, PROVO, UT 84602 USA

[2] BRITISH COLUMBIA CANC RES CTR, VANCOUVER, BC V5Z 1L3, CANADA

来源：

MUTATION RESEARCH-REVIEWS IN GENETIC TOXICOLOGY | 1995年 / 339卷 / 01期

关键词：

COMET ASSAY; SINGLE CELL GEL; MICROGEL ELECTROPHORESIS; DNA SINGLE STRAND BREAKS; DOUBLE STRAND BREAKS;

D O I：

10.1016/0165-1110(94)00013-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The comet assay is a sensitive and rapid method for DNA strand break detection in individual cells. Its use has increased significantly in the past few years. This paper is a review of the studies published to date that have made use of the comet assay. The principles of strand break detection using both the alkaline and neutral versions of the technique are discussed, and a basic methodology with currently used variations is presented. Applications in different fields are reviewed and possible future directions of the technique are briefly explored.

引用

页码：37 / 59

页数：23

共 159 条

[1] CALIBRATION OF PULSED FIELD GEL-ELECTROPHORESIS FOR MEASUREMENT OF DNA DOUBLE-STRAND BREAKS [J].