COMPLEX-FORMATION BETWEEN RAS AND RAF AND OTHER PROTEIN-KINASES

被引：458

作者：

VANAELST, L ^{[1
]}

BARR, M ^{[1
]}

MARCUS, S ^{[1
]}

POLVERINO, A ^{[1
]}

WIGLER, M ^{[1
]}

机构：

[1] COLD SPRING HARBOR LAB,POB 100,COLD SPRING HARBOR,NY 11724

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 13期

关键词：

SIGNAL TRANSDUCTION; CANCER; PROTEIN-PROTEIN INTERACTIONS; YEAST;

D O I：

10.1073/pnas.90.13.6213

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We used a Saccharomyces cerevisiae genetic system to detect the physical interaction of RAS and RAF oncoproteins. We also observed interaction between RAS and byr2, a protein kinase implicated as a mediator of the Schizo-saccharomyces pombe ras1 protein. Interaction with RAS required only the N-terminal domains of RAF or byr2 and was disrupted by mutations in either the guanine nucleotide-binding or effector-loop domains of RAS. We observed interaction between MEK (a kinase that phosphorylates mitogen-activated protein kinases) and the catalytic domain of RAF. RAS and MEK also interacted but only when RAF was overexpressed.

引用

页码：6213 / 6217

页数：5

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