CAUTIONARY NOTE FOR DMPO SPIN TRAPPING IN THE PRESENCE OF IRON-ION

被引：135

作者：

MAKINO, K ^{[1
]}

HAGIWARA, T ^{[1
]}

HAGI, A ^{[1
]}

NISHI, M ^{[1
]}

MURAKAMI, A ^{[1
]}

机构：

[1] SETSUNAN UNIV,FAC PHARMACEUT SCI,HIRAKATA,OSAKA 57301,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 172卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)91556-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

2-Hydroxy-5,5-dimethyl-1-pyrrolidinyloxy (DMPO-OH), which is known to be produced by spin trapping of hydroxyl radicals (·OH) with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) and has been a good monitor for detecting ·OH in biological systems, has been examined by EPR for its production scheme in the presence of iron ion. In an aqueous DMPO solution containing ferric ion (Fe3+), DMPO-OH was produced and addition of methanol, a good scavenger for ·OH, to this solution led to an aminoxyl radical, DMPO-OCH3, instead of DMPO-CH2OH which is produced by DMPO spin trapping of ·CH2OH arising from H-abstraction by ·OH. Also EPR measurements at 77K indicated the formation of a chelate between DMPO and Fe3+. Based on these, it has been elucidated that DMPO-OH as well as DMPO-OCH3 is formed by the nucleophilic attack of water and methanol to the chelating DMPO, respectively. © 1990.

引用

页码：1073 / 1080

页数：8

共 14 条

[1]

Mello Filho, Meneghini, Protection of mammalian cells by o-phenanthroline from lethal and DNA-damaging effects produced by active oxygen species, Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 847, pp. 82-89, (1985)

[2]

Carmichael, FEBS Lett, 261, pp. 165-170, (1990)

[3]

Krilov, Pifat, Herak, Electron spin resonance spin-trapping study of peroxidation of human low density lipoprotein, Canadian Journal of Chemistry, 66, pp. 1957-1960, (1988)

[4]

Janzen, Acc. Chem. Res, 4, pp. 31-40, (1971)

[5]

Pou, Hassett, Britigan, Cohen, Rosen, Anal. Biochem, 177, pp. 1-6, (1989)

[6]

Finkelstein, Rosen, Rauckman, Arch. Biochem. Biophys, 200, pp. 1-16, (1980)

[7]

Bonnett, Clark, Todd, 424. Experiments towards the synthesis of corrins. Part III. Formation of a bicyclic oxaziran from a ?1-pyroline and from the corresponding nitrone, Journal of the Chemical Society (Resumed), pp. 2102-2104, (1959)

[8]

Janzen, Free Radicals in Biology, 4, pp. 115-154, (1980)

[9]

Makino, Imaishi, Morinishi, Hagiwara, Takeuchi, Murakami, Free Rad. Res. Comms, 6, pp. 19-28, (1989)

[10]

Janzen, Liu, J. Magn. Reson, 9, pp. 510-512, (1973)

← 1 2 →