EARLY DISTURBANCE OF A MITOCHONDRIAL-DNA EXPRESSION IN GERBIL HIPPOCAMPUS AFTER TRANSIENT FOREBRAIN ISCHEMIA

The level of mRNA for cytochrome c oxidase subunit I (COX-I), which is encoded by mitochondrial DNA (mtDNA), progressively decreased in the hippocampal CA1 neurons of gerbils from 1-3 h of the reperfusion after 3.5 min of transient forebrain ischemia, and completely disappeared at 7 days. The activity of cytochrome c oxidase (COX) protein also showed the early decrease in the CA1 cells, and was followed by the reduction of the level of COX-1 DNA after 2 days. However, the activity of succinic dehydrogenase (SDH), a mitochondrial enzyme that is encoded by nuclear DNA, maintained normal activity until 1 day in the CA1 cells, and significantly decreased at 7 days. These results suggest that the early onset and the progressive disturbance of a mitochondrial DNA expression found selectively in the CA1 neurons could cause progressive failure of energy production of the cells that eventually results in the neuronal cell death.