MOLECULAR-DETECTION OF INTERFERON-ALPHA EXPRESSION IN MULTIPLE-SCLEROSIS BRAIN

被引：22

作者：

BRANDT, ER ^{[1
]}

MACKAY, IR ^{[1
]}

HERTZOG, PJ ^{[1
]}

CHEETHAM, BF ^{[1
]}

SHERRITT, M ^{[1
]}

BERNARD, CC ^{[1
]}

机构：

[1] LA TROBE UNIV,NEUROIMMUNOL LAB,BUNDOORA,VIC 3083,AUSTRALIA

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1993年 / 44卷 / 01期

关键词：

MULTIPLE SCLEROSIS; CYTOKINES AND DISEASES; INTERFERON-ALPHA EXPRESSION; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0165-5728(93)90261-V

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The demonstration of intermittent interferonaemia in patients with multiple sclerosis prompted a molecular analysis of brain tissue for expression of interferon-alpha genes. A sensitive method was developed based on the polymerase chain reaction. Primer sets were used that could amplify all interferons-alpha or two particular subtypes, interferon-alpha2 and interferon-alpha4. The procedure was successful in detecting expression of interferons-alpha in brain and non-brain tissues in most patients with multiple sclerosis. However, expression was demonstrable also in a similar proportion of patients with other neural diseases, and patients with other illnesses. The data indicate that there can be constitutive expression of interferons-alpha in brain tissue, but the possibility that this becomes amplified in multiple sclerosis was not revealed by this study.

引用

页码：1 / 6

页数：6

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