VASOACTIVE INTESTINAL PEPTIDE (VIP) - TISSUE DISTRIBUTION IN RAT AS MEASURED BY RADIOIMMUNOASSAY AND BY RADIORECEPTORASSAY

被引：74

作者：

BESSON, J ^{[1
]}

LABURTHE, M ^{[1
]}

BATAILLE, D ^{[1
]}

DUPONT, C ^{[1
]}

ROSSELIN, G ^{[1
]}

机构：

[1] HOP ST ANTOINE,INSERM,UNITE 55,UNITE RECH DIABETOL,F-75571 PARIS 12,FRANCE

来源：

ACTA ENDOCRINOLOGICA | 1978年 / 87卷 / 04期

关键词：

D O I：

10.1530/acta.0.0870799

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

This work was undertaken to study the distribution of VIP in the digestive tract. VIP was measured both by radioimmunoassay and by radioreceptorassay in order to determine whether immunoreactive VIP is related to a biologically active component. The effect of digestive extracts in inhibiting the binding of porcine [125I]VIP to the antibody (RIA) and to the rat liver plasma membranes (RRA) paralleled that of porcine VIP used as the standard. VIP was found throughout the digestive tract with especially high concentrations between the duodenum (1676 ± 186 ng/g) and the colon (1214 ± 214 ng/g); the maximal quantity occurred in the jejuno-ileum (11 698 ± 687 ng/g). Less than 1% of VIP was found in the epithelium whereas almost all VIP (> 99%) was localized in the mucosal muscular tissue of the jejunoileum. VIP concentration in the pancreas was 180 ± 26 ng/g of tissue. The VIP contents of the digestive tract were similar when measured either by radioimmunoassay or by radioreceptorassay. Immunoreactive VIP was found in the brain (155 ± 7 ng/g of tissue) and brain extracts competitively inhibited the binding of [125I]VIP (purified from gut) to liver plasma membranes. These results show that: 1) VIP from the overall gastrointestinal tract is a biological active molecule; 2) VIP from brain binds to receptors for intestinal VIP in liver.

引用

页码：799 / 810

页数：12

共 38 条

[1] INTESTINAL SECRETION - STIMULATION BY PEPTIDES [J].

BARBEZAT, GO ;

GROSSMAN, MI .

SCIENCE, 1971, 174 (4007) :422-+

[2] INTERACTIONS OF GLUCAGON, GUT GLUCAGON, VASOACTIVE INTESTINAL POLYPEPTIDE AND SECRETIN WITH LIVER AND FAT-CELL PLASMA-MEMBRANES - BINDING TO SPECIFIC SITES AND STIMULATION OF ADENYLATE CYCLASE [J].

BATAILLE, D ;

FREYCHET, P ;

ROSSELIN, G .

ENDOCRINOLOGY, 1974, 95 (03) :713-721

[3]

BATAILLE D, 1975, ISRAEL J MED SCI, V11, P687

[4]

BATAILLE D, 1977, GLUCAGON ITS ROLE PH

[5]

BATAILLE D, 1977, HORMONAL RECEPTORS D, P113

[6]

Bayliss WM, 1902, J PHYSIOL-LONDON, V28, P325

[7]

BLOOM SR, 1975, GUT, V16, P821

[8]

CHRISTOPHE JP, 1976, J BIOL CHEM, V251, P4629

[9] VASOACTIVE INTESTINAL POLYPEPTIDE AND GLUCAGON - STIMULATION OF ADENYLATE CYCLASE ACTIVITY VIA DISTINCT RECEPTORS IN LIVER AND FAT-CELL MEMBRANES [J].

DESBUQUOIS, B ;

LAUDAT, MH ;

LAUDAT, P .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1973, 53 (04) :1187-1194

[10]

DUPONT C, 1977, NOUV PRESSE MED, V6, P3752

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