DISRUPTION OF MITOTIC SPINDLE ORIENTATION IN A YEAST DYNEIN MUTANT

被引：344

作者：

LI, YY ^{[1
]}

YEH, E ^{[1
]}

HAYS, T ^{[1
]}

BLOOM, K ^{[1
]}

机构：

[1] UNIV MINNESOTA,DEPT GENET & CELL BIOL,ST PAUL,MN 55108

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 21期

关键词：

D O I：

10.1073/pnas.90.21.10096

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Dynein motor isoforms have been implicated as potential kinetochore-associated motors that power chromosome-to-pole movements during mitosis. The recent identification and sequence determination of genes encoding dynein isoforms has now permitted the in vivo analysis of dynein function in mitosis. In this report we describe the identification and mutational analysis of the gene, DHC1, encoding a dynein heavy chain isoform in Saccharomyces cerevisiae. Sequence analysis of a 9-kb genomic fragment of the DHC1 gene predicts a polypeptide highly homologous to dynein sequences characterized from sea urchin, Dictyostelium, Drosophila, and rat. Mutations in the yeast dynein gene disrupt the normal movement of the spindle into budding daughter cells but have no apparent effect on spindle assembly, spindle elongation, or chromosome segregation. Our results suggest that, in yeast, a dynein microtubul motor protein has a nonessential role in spindle assembly and chromosome movement but is involved in establishing the proper spindle orientation during cell division.

引用

页码：10096 / 10100

页数：5

共 19 条

[1] RELATIONSHIP OF ACTIN AND TUBULIN DISTRIBUTION TO BUD GROWTH IN WILD-TYPE AND MORPHOGENETIC-MUTANT SACCHAROMYCES-CEREVISIAE [J].