MOLECULAR-CLONING, EXPRESSION AND INSITU HYBRIDIZATION OF RAT-BRAIN GLUTAMIC-ACID DECARBOXYLASE MESSENGER-RNA

被引：68

作者：

JULIEN, JF

LEGAY, F

DUMAS, S

TAPPAZ, M

MALLET, J

机构：

[1] CNRS,NEUROBIOL CELLULAIRE & MOLEC LAB,F-91190 GIF SUR YVETTE,FRANCE

[2] HOP ST EUGENIE,CNRS,INSERM,U171,ST GENIS LAVAL,FRANCE

来源：

NEUROSCIENCE LETTERS | 1987年 / 73卷 / 02期

关键词：

Central nervous system; Fusion protein; Glutamate decarboxylase; In situ hybridization; mRNA cloning;

D O I：

10.1016/0304-3940(87)90013-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A cDNA library was generated in the expression vector λ GT11 from rat brain poly(A) + RNAs and screened with a GAD antiserum. Two clones reacted positively. One of them was shown to express a GAD activity which was specifically trapped on anti-GAD immunogel and was inhibited by γ-acetylenic-GABA. Blot hybridization analysis of RNAs from rat brain revealed a single 4 kilobases band. Preliminary in situ hybridizations showed numerous cells labelled by the GAD probe such as the Purkinje and stellate cells in the cerebellar cortex and the cells of the reticular thalamic nucleus. © 1987.

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页码：173 / 180

页数：8

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