A SENSITIVE RADIOIMMUNOASSAY MEASURING ENDOTHELIN-LIKE IMMUNOREACTIVITY IN HUMAN PLASMA - COMPARISON OF LEVELS IN PATIENTS WITH ESSENTIAL-HYPERTENSION AND NORMOTENSIVE CONTROL SUBJECTS

被引：220

作者：

DAVENPORT, AP ^{[1
]}

ASHBY, MJ ^{[1
]}

EASTON, P ^{[1
]}

ELLA, S ^{[1
]}

BEDFORD, J ^{[1
]}

DICKERSON, C ^{[1
]}

NUNEZ, DJ ^{[1
]}

CAPPER, SJ ^{[1
]}

BROWN, MJ ^{[1
]}

机构：

[1] AMERSHAM INT PLC,LIFE SCI BUSINESS,CARDIFF,WALES

来源：

CLINICAL SCIENCE | 1990年 / 78卷 / 03期

基金：

英国惠康基金;

关键词：

Endothelin; Hypertension; Radioimmunoassay;

D O I：

10.1042/cs0780261

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

1. A radioimmunoassay has been developed for measuring edothelin-like immunoreactivity in human plasma using an antibody raised against endothelin-1 which also cross-reacts with big edothelin-1 and endothelin-2 but not endothelin-3. 2. The sensitivity of the assay was 1 fmol/tube with inter- and intra-assay coefficients of variation of 13% and 9% respectively. Cross-reactivity with endothelin-3 and non-endothelin peptides was less than 1%. 3. Endothelin-like immunoreactivity was present in the plasma of hypertensive patients (n = 259) at a concentration of 5.7 ± 0.5 pmol/l (mean ± SEM), which was not significantly different from that of age-matched control subjects (5.1 ± 0.5 pmol/l). At these levels, endothelin-1 is unlikely to function as a circulating hormone. 4. Within the normotensive group, the concentration of endothelin-like immunoreactivity in plasma was positively correlated with mean arterial blood pressure, but in hypertensive patients it showed a significant negative correlation.

引用

页码：261 / 264

页数：4

共 17 条

[1] PRESENCE OF IMMUNOREACTIVE ENDOTHELIN IN HUMAN-PLASMA [J].