NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS

被引：7498

作者：

GAMBORG, OL

MILLER, RA

OJIMA, K

机构：

[1] Prairie Regional Laboratory, National Research Council of Canada, Saskatoon, Sask.

来源：

EXPERIMENTAL CELL RESEARCH | 1968年 / 50卷 / 01期

关键词：

D O I：

10.1016/0014-4827(68)90403-5

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2,4-d) has been designed. The cells required thiamine, 2,4-d and ammonium in addition to the usual mineral salts and sucrose. Optimum concentrations of nitrate and ammonium were 25 and 2 mM respectively. The highest yield of cells was achieved at an initial pH of 4.5-5.5. During the growth cycle the pH gradually increased to 6.0-6.2. © 1968.

引用

页码：151 / +

页数：1

共 17 条

[1]

CAREW DP, 1965, LLOYD, V28, P1

[2] CYTOKININ ACTIVATION OF THIAMINE BIOSYNTHESIS IN TOBACCO CALLUS CULTURES [J].