MUTUAL DEPENDENCE OF NA,K-ATPASE ALPHA-SUBUNITS AND BETA-SUBUNITS FOR CORRECT POSTTRANSLATIONAL PROCESSING AND INTRACELLULAR-TRANSPORT

被引：169

作者：

ACKERMANN, U

GEERING, K

机构：

[1] Institut de Pharmacologie de l'Université, CH-1005 Lausanne

来源：

FEBS LETTERS | 1990年 / 269卷 / 01期

关键词：

Glycosylation processing; Na; K-ATPase; Oocyte; Protein biosynthesis; Protein degradation; Protein oligomerization; Xenopus laevis;

D O I：

10.1016/0014-5793(90)81130-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we have followed the fate of newly synthesized α- and β-subunits of Na,K-ATPase in Xenopus oocytes injected with α and/or β cRNA to examine whether assembly of the two subunits is needed for a correct folding and/or for intracellular transport of Na,K-ATPase. Our data indicate that (1) assembly of α- and β-subunits occurs at the level of the ER, (2) β-subunits are needed for the newly synthesized α-subunit to adopt a stable configuration and (3) α- and β-subunits mutually depend on each other to be transported out of the ER. © 1990.

引用

页码：105 / 108

页数：4

共 15 条

[1] BINDING TO MEMBRANE-PROTEINS WITHIN THE ENDOPLASMIC-RETICULUM CANNOT EXPLAIN THE RETENTION OF THE GLUCOSE-REGULATED PROTEIN GRP78 IN XENOPUS OOCYTES [J].