CYTOPLASMIC BRIDGES AND GAP-JUNCTIONS IN AN INSECT CELL-LINE (AEDES-ALBOPICTUS)

Cell pairs of an insect cell line (Aedes albopictus, clone C6/36) were used to study simultaneously the diffusional and electrical properties of intercellular junctions. Diffusion studies involved injection of fluorescent molecules into one cell of a cell pair and visual inspection of their intercellular redistribution. Electrical measurements involved a dual voltage clamp method and whole-cell recording with patch pipettes. The voltage clamp protocol was aimed at examining the dependency of the junctional conductance, g(j), on membrane potential, V(m). Cell pairs exhibiting a voltage-dependent g(j) were found to allow intercellular diffusion of Lucifer Yellow CH (molecular mass, 443 Da), but not of FITC-dextran (molecular mass, 4400 Da). This response pattern is consistent with the presence of gap junctions in the intercellular junctions. Cell pairs showing no voltage dependence of g(j) were found to permit intercellular diffusion of both Lucifer Yellow CH and FITC-dextran (dextran labelled with fluorescein isothiocyanate). This behaviour is compatible with the presence of cytoplasmic bridges connecting the two adjacent cells. Hence, in culture the cells investigated express two kinds of intercellular structures, gap junctions and cytoplasmic bridges.