A METHOD FOR DETERMINATION OF RELATIVE SPECIFIC RADIOACTIVITY OF (3H) PROTEINS IN ACRYLAMIDE GEL

被引：57

作者：

CAIN, DF

PITNEY, RE

机构：

[1] Laboratories of Biochemistry, Department of Animal Biology, School of Veterinary Medicine, Philadelphia

来源：

ANALYTICAL BIOCHEMISTRY | 1968年 / 22卷 / 01期

关键词：

D O I：

10.1016/0003-2697(68)90253-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for discontinuous vertical gel electrophoresis of proteins at pH 8.9 in an ethylene diacrylate linked acrylamide gel. After staining the gel is segmented and each segment is dissolved in 1 M NH4OH and the protein-bound dye is measured colorimetrically. The NH4OH is evaporated and the samples are dissolved in a solubilizer and scintillation fluid and counted at an efficiency of 26-30% for tritium. © 1968.

引用

页码：11 / &

共 15 条

[1] SEPARATION OF CEREBROPROTEINS OF HUMAN BRAIN [J].