IL-2-DEPENDENT INVIVO AND INVITRO TYROSINE PHOSPHORYLATION OF IL-2 RECEPTOR GAMMA CHAIN

被引：40

作者：

ASAO, H ^{[1
]}

KUMAKI, S ^{[1
]}

TAKESHITA, T ^{[1
]}

NAKAMURA, M ^{[1
]}

SUGAMURA, K ^{[1
]}

机构：

[1] TOHOKU UNIV,SCH MED,DEPT MICROBIOL,2-1 SEIRYO MACHI,AOBA KU,SENDAI,MIYAGI 980,JAPAN

来源：

FEBS LETTERS | 1992年 / 304卷 / 2-3期

基金：

日本科学技术振兴机构;

关键词：

INTERLEUKIN-2; RECEPTOR; TYROSINE KINASE;

D O I：

10.1016/0014-5793(92)80605-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We previously reported a molecule, p64, which was tentatively named the gamma-chain, coprecipitable with the beta-chain of human interleukin-2 receptor (IL-2R). The present study demonstrated that the gamma-chain, as well as the beta-chain expressed on IL-2-responsive cells, is phosphorylated on tyrosine residues in an IL-2-dependent manner in vivo and in vitro. The in vivo tyrosine phosphorylation of both chains was similarly induced within 1 min after IL-2 stimulation, and their in vitro tyrosine phosphorylation with the anti-IL-2R-beta antibody-directed immunocomplex was also increased by treatment of cells with IL-2. These results suggest that a tyrosine kinase is associated with the beta-gamma subunit complex, of which activation by IL-2 may result in transduction of intracellular signals.

引用

页码：141 / 145

页数：5

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