CYCLIC-GMP MODULATORS AND EXCITOTOXIC INJURY IN CEREBRAL CORTICAL CULTURES

被引：47

作者：

LUSTIG, HS ^{[1
]}

VONBRAUCHITSCH, KL ^{[1
]}

CHAN, J ^{[1
]}

GREENBERG, DA ^{[1
]}

机构：

[1] SAN FRANCISCO GEN HOSP, SAN FRANCISCO, CA 94110 USA

来源：

BRAIN RESEARCH | 1992年 / 577卷 / 02期

关键词：

CYCLIC GMP; EXCITOTOXICITY; CELL CULTURE; N-METHYL-D-ASPARTATE; SODIUM NITROPRUSSIDE; GUANYLATE CYCLASE; PROTEIN KINASE; PHOSPHODIESTERASE;

D O I：

10.1016/0006-8993(92)90295-K

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

N-Methyl-D-aspartate (NMDA) receptor activation generates nitric oxide (NO) and cyclic GMP (cGMP) and produces 'excitotoxic' neuronal injury. To examine the possible role of cGMP in excitotoxicity, we evaluated the effects of agents that stimulate or inhibit cGMP activity on the release of lactate dehydrogenase from neuron-enriched cortical cultures. cGMP analogs exhibited no toxicity, and inhibitors of guanylate cyclase or of cGMP-dependent enzymes failed to protect cultures from the toxic effects of NMDA or the NO donor sodium nitroprusside. These findings argue against a role for cGMP in the pathogenesis of excitotoxic neuronal injury.

引用

页码：343 / 346

页数：4

共 29 条

[1] PRIMARY SEQUENCE OF CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE ISOZYMES AND THE DESIGN OF SELECTIVE INHIBITORS [J].