ANTIGENIC SUBUNIT OF THE POLYPEPTIDE ANTIGENIC COMPLEX OF THE MELVIN STRAIN OF NEISSERIA-GONORRHOEAE

An antigenic subunit of molecular weight 66,000 daltons has been isolated from the antigenic complex of the Melvin strain of Neisseria gonorrhoeae. Incubation of the complex in 8M urea at room temperature for four hours resulted in the dissociation of the subunit from the complex. It was separated from the complex by chromatography of the incubation mixture on a Sepharose 6B column in 50 mM ammonium bicarbonate pH 8.5 without 8M urea and further purified by affinity chromatography. This communication reports on a newly isolated antigenic protein devoid of LPS present in the bacteria. © 1979.