CRYSTALLIZATION AND PRELIMINARY-X-RAY CRYSTALLOGRAPHIC STUDIES OF UDP-N-ACETYLENOLPYRUVYLGLUCOSAMINE REDUCTASE

被引：15

作者：

BENSON, TE ^{[1
]}

WALSH, CT ^{[1
]}

HOGLE, JM ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,DEPT BIOL CHEM & MOLEC PHARMACOL,BOSTON,MA 02115

来源：

PROTEIN SCIENCE | 1994年 / 3卷 / 07期

关键词：

CRYSTALLIZATION; PEPTIDOGLYCAN; UDP-N-ACETYLENOL-PYRUVYLGLUCOSAMINE REDUCTASE; X-RAY CRYSTALLOGRAPHY;

D O I：

10.1002/pro.5560030718

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The overexpression and purification of the second enzyme in Escherichia coli peptidoglycan biosynthesis, UDP-N-acetylenolpyruvylglucosamine reductase (MurB), provided sufficient protein to undertake crystallization and X-ray crystallographic studies of the enzyme. MurB crystallizes in 14-20% PEG 8000, 100 mM sodium cacodylate, pH 8.0, and 200 mM calcium acetate in the presence of its substrate UDP-N-acetylglucosamine enolpyruvate. Crystals of MurB belong to the tetragonal space group P4(1)2(1)2 with a = b = 49.6 Angstrom, c = 263.2 Angstrom, and alpha = beta = gamma = 90 degrees at -160 degrees C and diffract to at least 2.5 Angstrom. Screening for heavy atom derivatives has yielded a single site that is reactive with both methylmercury nitrate and Thimerosal.

引用

页码：1125 / 1127

页数：3

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