COMPLEMENTARY OLIGONUCLEOTIDE SEQUENCE INHIBITS BOTH VMW65 GENE-EXPRESSION AND REPLICATION OF HERPES-SIMPLEX VIRUS

被引：14

作者：

DRAPER, KG ^{[1
]}

CERUZZI, M ^{[1
]}

KMETZ, ME ^{[1
]}

STURZENBECKER, LJ ^{[1
]}

机构：

[1] SCHERING PLOUGH CORP,DEPT ANTIVIRAL CHEMOTHERAPY,BLOOMFIELD,NJ 07003

来源：

ANTIVIRAL RESEARCH | 1990年 / 13卷 / 04期

关键词：

Herpes simplex virus; Oligonucleotide inhibition;

D O I：

10.1016/0166-3542(90)90034-5

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The virion tegument protein, Vmw65, of herpes simplex virus is a transacting molecule which induces immediate early gene transcription. We show that an oligodeoxyribonucleotide which is complementary to the translation initiation region of Vmw65 mRNA inhibited the expression of Vmw65 biological activity in a Vmw65-expressing cell line and reduced the yield of HSV-1 in tissue culture. The levels of oligomer required to effect viral replication resulted in no observable cellular toxicity. © 1990.

引用

页码：151 / 164

页数：14

共 21 条

[1] ANTISENSE RNA INHIBITS ENDOGENOUS GENE-EXPRESSION IN MOUSE PREIMPLANTATION EMBRYOS - LACK OF DOUBLE-STRANDED-RNA MELTING ACTIVITY [J].

BEVILACQUA, A ;

ERICKSON, RP ;

HIEBER, V .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (03) :831-835

[2] INHIBITION OF RABBIT GLOBIN MESSENGER-RNA TRANSLATION BY SEQUENCE-SPECIFIC OLIGODEOXYRIBONUCLEOTIDES [J].

BLAKE, KR ;

MURAKAMI, A ;

MILLER, PS .

BIOCHEMISTRY, 1985, 24 (22) :6132-6138

[3] IDENTIFICATION OF HERPES-SIMPLEX VIRUS-DNA SEQUENCES WHICH ENCODE A TRANS-ACTING POLYPEPTIDE RESPONSIBLE FOR STIMULATION OF IMMEDIATE EARLY TRANSCRIPTION [J].

CAMPBELL, MEM ;

PALFREYMAN, JW ;

PRESTON, CM .

JOURNAL OF MOLECULAR BIOLOGY, 1984, 180 (01) :1-19

[4] THE INTRACELLULAR AND EXTRACELLULAR FATE OF OLIGODEOXYRIBONUCLEOTIDES IN TISSUE-CULTURE SYSTEMS [J].

CERUZZI, M ;

DRAPER, K .

NUCLEOSIDES & NUCLEOTIDES, 1989, 8 (5-6) :815-818

[5] DNA-SEQUENCE OF THE HERPES-SIMPLEX VIRUS TYPE-1 GENE WHOSE PRODUCT IS RESPONSIBLE FOR TRANSCRIPTIONAL ACTIVATION OF IMMEDIATE EARLY PROMOTERS [J].

DALRYMPLE, MA ;

MCGEOCH, DJ ;

DAVISON, AJ ;

PRESTON, CM .

NUCLEIC ACIDS RESEARCH, 1985, 13 (21) :7865-7879

[6] TEMPERATURE-SENSITIVE MUTANTS OF REOVIRUS TYPE 3 FEATURES OF GENETIC RECOMBINATION [J].

FIELDS, BN .

VIROLOGY, 1971, 46 (01) :142-+

[7] NEW TECHNIQUE FOR ASSAY OF INFECTIVITY OF HUMAN ADENOVIRUS 5 DNA [J].

GRAHAM, FL ;

VANDEREB, AJ .

VIROLOGY, 1973, 52 (02) :456-467

[8] HERPES-SIMPLEX VIRUS MESSENGER-RNA SPECIES MAPPING IN ECORI FRAGMENT-I [J].

HALL, LM ;

DRAPER, KG ;

FRINK, RJ ;

COSTA, RH ;

WAGNER, EK .

JOURNAL OF VIROLOGY, 1982, 43 (02) :594-607

[9] PROTEINS SPECIFIED BY HERPES-SIMPLEX VIRUS .2. VIRION POLYPEPTIDES OF TYPE 1 STRAINS [J].

HEINE, JW ;

HONESS, RW ;

CASSAI, E ;

ROIZMAN, B .

JOURNAL OF VIROLOGY, 1974, 14 (03) :640-651

[10] INHIBITION OF THYMIDINE KINASE GENE-EXPRESSION BY ANTI-SENSE RNA - A MOLECULAR APPROACH TO GENETIC-ANALYSIS [J].

IZANT, JG ;

WEINTRAUB, H .

CELL, 1984, 36 (04) :1007-1015

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