RADIOIMMUNOASSAY OF THE INSULIN RECEPTOR - NEW PROBE OF RECEPTOR STRUCTURE AND FUNCTION

被引：57

作者：

HARRISON, LC

FLIER, J

ITIN, J

KAHN, CR

ROTH, J

机构：

[1] Diabetes Branch, National Institute of Arthritis, Metabolism, and Infectious Diseases, National Institutes of Health, Bethesda

来源：

SCIENCE | 1979年 / 203卷 / 4380期

关键词：

D O I：

10.1126/science.83675

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A sensitive and specific radioimmunoassay for the insulin receptor has been developed employing receptor autoantibodies from the serum of a patient with insulin-resistant diabetes. The assay detects insulin binding sites at concentrations as low as 0.1 nanomolar; distinguishes between receptors originating from human placental membranes, human lymphoblastoid cells, and mouse liver membranes; and measures the receptor independently of its binding function. Down-regulation, or loss of binding after exposure to insulin, is associated with loss of immunoreactive receptor. Copyright © 1979 AAAS.

引用

页码：544 / 547

页数：4

共 14 条

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[10] ANTI-INSULIN RECEPTOR ANTIBODIES INHIBIT INSULIN BINDING AND STIMULATE GLUCOSE-METABOLISM IN SKELETAL-MUSCLE [J].

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DIABETOLOGIA, 1978, 14 (05) :311-317

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