REVERSE-TRANSCRIPTASE ENCODED BY A HUMAN TRANSPOSABLE ELEMENT

被引：623

作者：

MATHIAS, SL

SCOTT, AF

KAZAZIAN, HH

BOEKE, JD

GABRIEL, A ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV, SCH MED, DEPT MOLEC BIOL & GENET, BALTIMORE, MD 21205 USA

[2] JOHNS HOPKINS UNIV, SCH MED, DEPT MED, CTR MED GENET, BALTIMORE, MD 21205 USA

[3] JOHNS HOPKINS UNIV, SCH MED, DEPT PEDIAT, CTR MED GENET, BALTIMORE, MD 21205 USA

来源：

SCIENCE | 1991年 / 254卷 / 5039期

关键词：

D O I：

10.1126/science.1722352

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

L1 elements are highly repeated mammalian DNA sequences whose structure suggests dispersal by retrotransposition. A consensus L1 clement encodes a protein with sequence similarity to known reverse transcriptases. The second open reading frame from the human L1 element L1.2A was expressed as a fusion protein targeted to Tyl virus-like particles in Saccharomyces cerevisiae and shown to have reverse transcriptase activity. This activity was eliminated by a missense mutation in the highly conserved amino acid motif Y/F-X-D-D. Thus, L1 represents a potential source of the reverse transcriptase activity necessary for dispersion of the many classes of mammalian retroelements.

引用

页码：1808 / 1810

页数：3

共 41 条

[1] THE FUNCTIONS AND RELATIONSHIPS OF TY-VLP PROTEINS IN YEAST REFLECT THOSE OF MAMMALIAN RETROVIRAL PROTEINS [J].